I've just re-watched Ed Wood, and was gobsmacked when I noticed the Sarah Jessica Parker character reading a newspaper review and saying "Do I really look like a horse?". I can't believe Tim Burton got her to say that. What a guy.

Any other examples? The thing is, it's no fun when the butt of the joke is complicit in it, and publicly being a 'good sport' (a la Extras). Which is, I admit, a possibility with SJP in Ed Wood.

But are there any great examples of this sort of thing when the victim most probably isn't in on the joke?

I remember Tortelvis getting Gloria Hunniford to say "I'll kiss your ring later"...

Supposedly Margaret Dumont was never in on any of the gags at her expense in the Marx Brothers' movies.

Tim Burton seems to have some form in this. After all he did get Charlton Heston to warn another charactor about the danger of guns in the remake of The Planet of the Apes.